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  Home::In vitro Antibacterial Activities of Berry Fruits (Citrus aurantifolia and Citrus sinensis) against
  In vitro Antibacterial Activities of Berry Fruits (Citrus aurantifolia and Citrus sinensis) against

Berry fruits are rich sources of bioactive compounds, such as phenolics and organic acids, which have antimicrobial activities against human pathogens. Present investigation focused on the antibacterial activities of the aqueous, ethanol, butanol and trichloroacetic acid extracts of Citrus aurentifolia (Lime fruit) and Citrus sinensis (Sweet orange) against four Gram negative bacterial pathogens (Escherichia coli are predominant followed by Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis) isolated from the urine specimens. The extracts were tested invitro by agar well diffusion, disc diffusion and minimum inhibitory concentration (MIC) methods. It has been observed that TCA and butanol extracts of both fruit showed potential antibacterial activity, whereas ethanol and aqueous extracts showed least antibacterial activity against the tested pathogens. Lime (Citrus aurantifolia) was found to be most effective. Klebsiella pneumoniae and Pseudomonas aeruginosa were found to be more susceptible for both the extracts (TCA and butanol) of lime fruit. The Minimum Inhibitory Concentration for TCA extract against Klebsiella pneumoniae and Pseudomonas aeruginosa was found to be 50g and 100g respectively. TLC-Bioautography was performed in order to indicate the presence of active compounds in TCA extract of lime fruit which were found to be very effective against Klebsiella pneumoniae. The result revealed that compounds eluted at Rf 0.91 for TCA extract exhibited strong antibacterial activity. Findings from this study show that the ethnomedical use of the fruit as antibacterial remedy is well placed and further effort is warranted; in order to isolate and elucidate the active principles in the fruit with the view of deriving the potential therapeutic benefits inherent in Citrus aurantifolia.

Date: 15, Sep 2012 Posted By: admin
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