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  Home::Phenotypic detection of extended-spectrum beta-lactamase producing Pseudomonas aeruginosa from Hospi
  Phenotypic detection of extended-spectrum beta-lactamase producing Pseudomonas aeruginosa from Hospi

Resistance to expanded-spectrum cephalosporins in Pseudomonas aeruginosa and other Gram-negative bacteria is mediated by extended-spectrum beta lactamases (ESBLs). ESBL-producing bacteria have been responsible for clinical failures and several outbreaks in hospitals when not accurately and promptly detected. The purpose of this study was to phenotypically detect the production of ESBLs among 54 multidrug resistant clinical isolates of P. aeruginosa obtained from various specimens in 5 hospitals in Southwest Nigeria. The presence of ESBL was determined by double disk synergy test (DDST) using amoxicillin/clavulanate as the ESBL inhibitor. Of the 54 isolates, 5 (9.3%) displayed a clear production of ESBL by showing a synergy towards the clavulanic acid. A low rate of detection of ESBL by DDST despite a high cephalosporin resistance rate of 53.7% observed in this study suggests the presence of other β-lactamases not easily detected phenotypically by DDST. Consequently, a combination of phenotypic and molecular detection methods would be essential for a reliable epidemiological investigation of the diverse groups of ESBLs produced by Pseudomonas aeruginosa and other Gram-negative bacteria. Routine surveillance of antimicrobial resistance and robust detection of ESBL among strains of P. aeruginosa is clinically and epidemiologically important to forestall rapid spread and transfer of resistance and ESBL genes among other nosocomial pathogens.

Date: 10, Oct 2012 Posted By: admin
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